Construct: shRNA TRCN0000363004

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027067.1-528s21c1
DNA Barcode:
CATTCGTGAAGGATATGTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
H2bfm (69389)
Gene Description:
H2B histone family, member M
Transcript:
RefSeq NM_027067.1 (NON-CURRENT)
Match location:
Position 528 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363004)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTCGTGAAGGATATGTTTG
Hairpin Sequence:
5'-CCGG-CATTCGTGAAGGATATGTTTG-CTCGAG-CAAACATATCCTTCACGAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTCGTGAAGGATATGTTTGCTCGAGCAAACATATCCTTCACGAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTCGTGAAGGATATGTTTGCTCGAGCAAACATATCCTTCACGAATG-3'

Other clones with same target sequence:

(none)