Construct: shRNA TRCN0000363019

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027472.2-513s21c1
DNA Barcode:
GCTTGAACTGGACAAGTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
5730455P16Rik (70591)
Gene Description:
RIKEN cDNA 5730455P16 gene
Transcript:
RefSeq NM_027472.2 (NON-CURRENT)
Match location:
Position 513 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363019)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGAACTGGACAAGTATAT
Hairpin Sequence:
5'-CCGG-GCTTGAACTGGACAAGTATAT-CTCGAG-ATATACTTGTCCAGTTCAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGAACTGGACAAGTATATCTCGAGATATACTTGTCCAGTTCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGAACTGGACAAGTATATCTCGAGATATACTTGTCCAGTTCAAGC-3'

Other clones with same target sequence:

(none)