Construct: shRNA TRCN0000363030

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028004.2-5069s21c1
DNA Barcode:
CCGGAAAGAAAGCTGATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttn (22138)
Gene Description:
titin
Transcript:
RefSeq NM_028004.2 (CURRENT)
Match location:
Position 5069 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363030)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAAAGAAAGCTGATTATT
Hairpin Sequence:
5'-CCGG-CCGGAAAGAAAGCTGATTATT-CTCGAG-AATAATCAGCTTTCTTTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAAAGAAAGCTGATTATTCTCGAGAATAATCAGCTTTCTTTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAAAGAAAGCTGATTATTCTCGAGAATAATCAGCTTTCTTTCCGG-3'

Other clones with same target sequence:

(none)