Construct: shRNA TRCN0000363196

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000845.2-2118s21c1
DNA Barcode:
GTGACCTTTGTCCGCTATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRM8 (2918)
Gene Description:
glutamate metabotropic receptor 8
Transcript:
RefSeq NM_000845.2 (NON-CURRENT)
Match location:
Position 2118 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363196)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGACCTTTGTCCGCTATAAT
Hairpin Sequence:
5'-CCGG-GTGACCTTTGTCCGCTATAAT-CTCGAG-ATTATAGCGGACAAAGGTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGACCTTTGTCCGCTATAATCTCGAGATTATAGCGGACAAAGGTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGACCTTTGTCCGCTATAATCTCGAGATTATAGCGGACAAAGGTCAC-3'

Other clones with same target sequence:

(none)