Construct: shRNA TRCN0000363219

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017506.1-682s21c1
DNA Barcode:
TCTGTATTCCTTGCTACAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR7A5 (26659)
Gene Description:
olfactory receptor family 7 subfamily A member 5
Transcript:
RefSeq NM_017506.1 (NON-CURRENT)
Match location:
Position 682 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363219)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGTATTCCTTGCTACAAAT
Hairpin Sequence:
5'-CCGG-TCTGTATTCCTTGCTACAAAT-CTCGAG-ATTTGTAGCAAGGAATACAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGTATTCCTTGCTACAAATCTCGAGATTTGTAGCAAGGAATACAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGTATTCCTTGCTACAAATCTCGAGATTTGTAGCAAGGAATACAGA-3'

Other clones with same target sequence:

(none)