Construct: shRNA TRCN0000363244

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005609.1-735s21c1
DNA Barcode:
GGAGCAGATGGCCCAGTTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EDA (1896)
Gene Description:
ectodysplasin A
Transcript:
RefSeq NM_001005609.1 (NON-CURRENT)
Match location:
Position 735 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363244)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGCAGATGGCCCAGTTAAA
Hairpin Sequence:
5'-CCGG-GGAGCAGATGGCCCAGTTAAA-CTCGAG-TTTAACTGGGCCATCTGCTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGCAGATGGCCCAGTTAAACTCGAGTTTAACTGGGCCATCTGCTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGCAGATGGCCCAGTTAAACTCGAGTTTAACTGGGCCATCTGCTCC-3'

Other clones with same target sequence:

(none)