Construct: shRNA TRCN0000363278
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001004134.1-122s21c1
- DNA Barcode:
- TGAATGGCCTCATCATCTTTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- OR10AD1 (121275)
- Gene Description:
- olfactory receptor family 10 subfamily AD member 1
- Transcript:
- RefSeq NM_001004134.1 (CURRENT)
- Match location:
- Position 122 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000363278)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TGAATGGCCTCATCATCTTTA
- Hairpin Sequence:
-
5'-CCGG-TGAATGGCCTCATCATCTTTA-CTCGAG-TAAAGATGATGAGGCCATTCA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTGAATGGCCTCATCATCTTTACTCGAGTAAAGATGATGAGGCCATTCATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATGAATGGCCTCATCATCTTTACTCGAGTAAAGATGATGAGGCCATTCA-3'
Other clones with same target sequence:
(none)
