Construct: shRNA TRCN0000363284

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004134.1-14s21c1
DNA Barcode:
GCAGCATAGTGACGGAATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR10AD1 (121275)
Gene Description:
olfactory receptor family 10 subfamily AD member 1
Transcript:
RefSeq NM_001004134.1 (CURRENT)
Match location:
Position 14 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363284)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCATAGTGACGGAATTTA
Hairpin Sequence:
5'-CCGG-GCAGCATAGTGACGGAATTTA-CTCGAG-TAAATTCCGTCACTATGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCATAGTGACGGAATTTACTCGAGTAAATTCCGTCACTATGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCATAGTGACGGAATTTACTCGAGTAAATTCCGTCACTATGCTGC-3'

Other clones with same target sequence:

(none)