Construct: shRNA TRCN0000363351

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004686.4-1327s21c1
DNA Barcode:
TGAAGGGCTTCATGGTATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTMR7 (9108)
Gene Description:
myotubularin related protein 7
Transcript:
RefSeq NM_004686.4 (NON-CURRENT)
Match location:
Position 1327 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363351)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAGGGCTTCATGGTATTAA
Hairpin Sequence:
5'-CCGG-TGAAGGGCTTCATGGTATTAA-CTCGAG-TTAATACCATGAAGCCCTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAGGGCTTCATGGTATTAACTCGAGTTAATACCATGAAGCCCTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAGGGCTTCATGGTATTAACTCGAGTTAATACCATGAAGCCCTTCA-3'

Other clones with same target sequence:

(none)