Construct: shRNA TRCN0000363592

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025907.3-1046s21c1
DNA Barcode:
CCAGTGACTCTGCTTCACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mettl6 (67011)
Gene Description:
methyltransferase like 6
Transcript:
RefSeq NM_025907.3 (CURRENT)
Match location:
Position 1046 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363592)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGACTCTGCTTCACTTT
Hairpin Sequence:
5'-CCGG-CCAGTGACTCTGCTTCACTTT-CTCGAG-AAAGTGAAGCAGAGTCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGACTCTGCTTCACTTTCTCGAGAAAGTGAAGCAGAGTCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGACTCTGCTTCACTTTCTCGAGAAAGTGAAGCAGAGTCACTGG-3'

Other clones with same target sequence:

  1. TRCN0000097558