Construct: shRNA TRCN0000363636
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_004787.1-1153s21c1
- DNA Barcode:
- CCTGGAGCTTTCTCACCATAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SLIT2 (9353)
- Gene Description:
- slit guidance ligand 2
- Transcript:
- RefSeq NM_004787.1 (NON-CURRENT)
- Match location:
- Position 1153 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000363636)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTGGAGCTTTCTCACCATAT
- Hairpin Sequence:
-
5'-CCGG-CCTGGAGCTTTCTCACCATAT-CTCGAG-ATATGGTGAGAAAGCTCCAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTGGAGCTTTCTCACCATATCTCGAGATATGGTGAGAAAGCTCCAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTGGAGCTTTCTCACCATATCTCGAGATATGGTGAGAAAGCTCCAGG-3'
Other clones with same target sequence:
-
TRCN0000056312
