Construct: shRNA TRCN0000363645

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009409.2-1891s21c1
DNA Barcode:
CCGCCAAATCTCTAGCTGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Top2b (21974)
Gene Description:
topoisomerase (DNA) II beta
Transcript:
RefSeq NM_009409.2 (CURRENT)
Match location:
Position 1891 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363645)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCCAAATCTCTAGCTGTAT
Hairpin Sequence:
5'-CCGG-CCGCCAAATCTCTAGCTGTAT-CTCGAG-ATACAGCTAGAGATTTGGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCCAAATCTCTAGCTGTATCTCGAGATACAGCTAGAGATTTGGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCCAAATCTCTAGCTGTATCTCGAGATACAGCTAGAGATTTGGCGG-3'

Other clones with same target sequence:

  1. TRCN0000070989