Construct: shRNA TRCN0000363776

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018737.4-223s21c1
DNA Barcode:
GCTGGAACTTTCTCACCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctps2 (55936)
Gene Description:
cytidine 5'-triphosphate synthase 2
Transcript:
RefSeq NM_018737.4 (NON-CURRENT)
Match location:
Position 223 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363776)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGAACTTTCTCACCTTAT
Hairpin Sequence:
5'-CCGG-GCTGGAACTTTCTCACCTTAT-CTCGAG-ATAAGGTGAGAAAGTTCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGAACTTTCTCACCTTATCTCGAGATAAGGTGAGAAAGTTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGAACTTTCTCACCTTATCTCGAGATAAGGTGAGAAAGTTCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000075989