Construct: shRNA TRCN0000363838

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133752.2-606s21c1
DNA Barcode:
GCCTGACTTTATATGGGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Opa1 (74143)
Gene Description:
OPA1, mitochondrial dynamin like GTPase
Transcript:
RefSeq NM_133752.2 (NON-CURRENT)
Match location:
Position 606 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363838)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGACTTTATATGGGAAAT
Hairpin Sequence:
5'-CCGG-GCCTGACTTTATATGGGAAAT-CTCGAG-ATTTCCCATATAAAGTCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGACTTTATATGGGAAATCTCGAGATTTCCCATATAAAGTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGACTTTATATGGGAAATCTCGAGATTTCCCATATAAAGTCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000091110