Construct: shRNA TRCN0000363862

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001144949.1-519s21c1
DNA Barcode:
ATGTGACTCTAACTGTCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spocd1 (622480)
Gene Description:
SPOC domain containing 1
Transcript:
RefSeq NM_001144949.1 (NON-CURRENT)
Match location:
Position 519 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363862)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTGACTCTAACTGTCAAAT
Hairpin Sequence:
5'-CCGG-ATGTGACTCTAACTGTCAAAT-CTCGAG-ATTTGACAGTTAGAGTCACAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTGACTCTAACTGTCAAATCTCGAGATTTGACAGTTAGAGTCACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTGACTCTAACTGTCAAATCTCGAGATTTGACAGTTAGAGTCACAT-3'

Other clones with same target sequence:

(none)