Construct: shRNA TRCN0000363917

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011220.2-437s21c1
DNA Barcode:
ATAGGTCTTAGGGTTGCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pts (19286)
Gene Description:
6-pyruvoyl-tetrahydropterin synthase
Transcript:
RefSeq NM_011220.2 (NON-CURRENT)
Match location:
Position 437 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363917)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATAGGTCTTAGGGTTGCTATT
Hairpin Sequence:
5'-CCGG-ATAGGTCTTAGGGTTGCTATT-CTCGAG-AATAGCAACCCTAAGACCTAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATAGGTCTTAGGGTTGCTATTCTCGAGAATAGCAACCCTAAGACCTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATAGGTCTTAGGGTTGCTATTCTCGAGAATAGCAACCCTAAGACCTAT-3'

Other clones with same target sequence:

(none)