Construct: shRNA TRCN0000363955

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001162910.1-2231s21c1
DNA Barcode:
ACATTGACAGTGATCTCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm3604 (100041979)
Gene Description:
predicted gene 3604
Transcript:
RefSeq NM_001162910.1 (CURRENT)
Match location:
Position 2231 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000363955)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATTGACAGTGATCTCATAA
Hairpin Sequence:
5'-CCGG-ACATTGACAGTGATCTCATAA-CTCGAG-TTATGAGATCACTGTCAATGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATTGACAGTGATCTCATAACTCGAGTTATGAGATCACTGTCAATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATTGACAGTGATCTCATAACTCGAGTTATGAGATCACTGTCAATGT-3'

Other clones with same target sequence:

(none)