Construct: shRNA TRCN0000364199

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198865.1-1925s21c1
DNA Barcode:
CCTCCTGCACCTGGGTAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slitrk5 (75409)
Gene Description:
SLIT and NTRK-like family, member 5
Transcript:
RefSeq NM_198865.1 (CURRENT)
Match location:
Position 1925 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000364199)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCTGCACCTGGGTAATAA
Hairpin Sequence:
5'-CCGG-CCTCCTGCACCTGGGTAATAA-CTCGAG-TTATTACCCAGGTGCAGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCTGCACCTGGGTAATAACTCGAGTTATTACCCAGGTGCAGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCTGCACCTGGGTAATAACTCGAGTTATTACCCAGGTGCAGGAGG-3'

Other clones with same target sequence:

(none)