Construct: shRNA TRCN0000364259

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133643.3-830s21c1
DNA Barcode:
CGATCAGGACTTGCTAGATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Edaradd (171211)
Gene Description:
EDAR (ectodysplasin-A receptor)-associated death domain
Transcript:
RefSeq NM_133643.3 (NON-CURRENT)
Match location:
Position 830 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000364259)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATCAGGACTTGCTAGATAC
Hairpin Sequence:
5'-CCGG-CGATCAGGACTTGCTAGATAC-CTCGAG-GTATCTAGCAAGTCCTGATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATCAGGACTTGCTAGATACCTCGAGGTATCTAGCAAGTCCTGATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATCAGGACTTGCTAGATACCTCGAGGTATCTAGCAAGTCCTGATCG-3'

Other clones with same target sequence:

(none)