Construct: shRNA TRCN0000364286

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173851.2-1140s21c1
DNA Barcode:
CACATCTGGTCTCTAACAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC30A8 (169026)
Gene Description:
solute carrier family 30 member 8
Transcript:
RefSeq NM_173851.2 (NON-CURRENT)
Match location:
Position 1140 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000364286)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACATCTGGTCTCTAACAATG
Hairpin Sequence:
5'-CCGG-CACATCTGGTCTCTAACAATG-CTCGAG-CATTGTTAGAGACCAGATGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACATCTGGTCTCTAACAATGCTCGAGCATTGTTAGAGACCAGATGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACATCTGGTCTCTAACAATGCTCGAGCATTGTTAGAGACCAGATGTG-3'

Other clones with same target sequence:

(none)