Construct: shRNA TRCN0000364308

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000457.3-153s21c1
DNA Barcode:
GCCTACACCACCCTGGAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HNF4A (3172)
Gene Description:
hepatocyte nuclear factor 4 alpha
Transcript:
RefSeq NM_000457.3 (NON-CURRENT)
Match location:
Position 153 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000364308)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTACACCACCCTGGAATTT
Hairpin Sequence:
5'-CCGG-GCCTACACCACCCTGGAATTT-CTCGAG-AAATTCCAGGGTGGTGTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTACACCACCCTGGAATTTCTCGAGAAATTCCAGGGTGGTGTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTACACCACCCTGGAATTTCTCGAGAAATTCCAGGGTGGTGTAGGC-3'

Other clones with same target sequence:

(none)