Construct: shRNA TRCN0000364349

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182920.1-3272s21c1
DNA Barcode:
GTTTGGTGAAGATCGATTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADAMTS9 (56999)
Gene Description:
ADAM metallopeptidase with thrombospondin type 1 motif 9
Transcript:
RefSeq NM_182920.1 (NON-CURRENT)
Match location:
Position 3272 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000364349)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTGGTGAAGATCGATTAAA
Hairpin Sequence:
5'-CCGG-GTTTGGTGAAGATCGATTAAA-CTCGAG-TTTAATCGATCTTCACCAAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTGGTGAAGATCGATTAAACTCGAGTTTAATCGATCTTCACCAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTGGTGAAGATCGATTAAACTCGAGTTTAATCGATCTTCACCAAAC-3'

Other clones with same target sequence:

(none)