Construct: shRNA TRCN0000364466

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024738.1-321s21c1
DNA Barcode:
GCAGTTTAACTTGGGCAATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C12orf49 (79794)
Gene Description:
chromosome 12 open reading frame 49
Transcript:
RefSeq NM_024738.1 (NON-CURRENT)
Match location:
Position 321 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000364466)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTTAACTTGGGCAATAG
Hairpin Sequence:
5'-CCGG-GCAGTTTAACTTGGGCAATAG-CTCGAG-CTATTGCCCAAGTTAAACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTTAACTTGGGCAATAGCTCGAGCTATTGCCCAAGTTAAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTTAACTTGGGCAATAGCTCGAGCTATTGCCCAAGTTAAACTGC-3'

Other clones with same target sequence:

(none)