Construct: shRNA TRCN0000364532

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020806.4-1215s21c1
DNA Barcode:
GAAGACCGCAGTGGGATAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPHN (10243)
Gene Description:
gephyrin
Transcript:
RefSeq NM_020806.4 (CURRENT)
Match location:
Position 1215 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000364532)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGACCGCAGTGGGATAAAT
Hairpin Sequence:
5'-CCGG-GAAGACCGCAGTGGGATAAAT-CTCGAG-ATTTATCCCACTGCGGTCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGACCGCAGTGGGATAAATCTCGAGATTTATCCCACTGCGGTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGACCGCAGTGGGATAAATCTCGAGATTTATCCCACTGCGGTCTTC-3'

Other clones with same target sequence:

  1. TRCN0000313416