Construct: shRNA TRCN0000364578

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022123.2-1198s21c1
DNA Barcode:
CAGTGCCACCATAGCTATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPAS3 (64067)
Gene Description:
neuronal PAS domain protein 3
Transcript:
RefSeq NM_022123.2 (NON-CURRENT)
Match location:
Position 1198 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000364578)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTGCCACCATAGCTATTAA
Hairpin Sequence:
5'-CCGG-CAGTGCCACCATAGCTATTAA-CTCGAG-TTAATAGCTATGGTGGCACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTGCCACCATAGCTATTAACTCGAGTTAATAGCTATGGTGGCACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTGCCACCATAGCTATTAACTCGAGTTAATAGCTATGGTGGCACTG-3'

Other clones with same target sequence:

(none)