Construct: shRNA TRCN0000364598

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007158.4-1356s21c1
DNA Barcode:
TAGAGCGAGCAACCAATATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSDE1 (7812)
Gene Description:
cold shock domain containing E1
Transcript:
RefSeq NM_007158.4 (NON-CURRENT)
Match location:
Position 1356 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000364598)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGAGCGAGCAACCAATATAG
Hairpin Sequence:
5'-CCGG-TAGAGCGAGCAACCAATATAG-CTCGAG-CTATATTGGTTGCTCGCTCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGAGCGAGCAACCAATATAGCTCGAGCTATATTGGTTGCTCGCTCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGAGCGAGCAACCAATATAGCTCGAGCTATATTGGTTGCTCGCTCTA-3'

Other clones with same target sequence:

(none)