Construct: shRNA TRCN0000364646

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018719.4-1129s21c1
DNA Barcode:
GCCATAACTGTTCGAGATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDCA7L (55536)
Gene Description:
cell division cycle associated 7 like
Transcript:
RefSeq NM_018719.4 (NON-CURRENT)
Match location:
Position 1129 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000364646)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATAACTGTTCGAGATAAA
Hairpin Sequence:
5'-CCGG-GCCATAACTGTTCGAGATAAA-CTCGAG-TTTATCTCGAACAGTTATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATAACTGTTCGAGATAAACTCGAGTTTATCTCGAACAGTTATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATAACTGTTCGAGATAAACTCGAGTTTATCTCGAACAGTTATGGC-3'

Other clones with same target sequence:

(none)