Construct: shRNA TRCN0000364784

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032860.3-817s21c1
DNA Barcode:
AGAGTCGCTTCACGGAGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LTV1 (84946)
Gene Description:
LTV1 ribosome biogenesis factor
Transcript:
RefSeq NM_032860.3 (NON-CURRENT)
Match location:
Position 817 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000364784)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGTCGCTTCACGGAGTATT
Hairpin Sequence:
5'-CCGG-AGAGTCGCTTCACGGAGTATT-CTCGAG-AATACTCCGTGAAGCGACTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGTCGCTTCACGGAGTATTCTCGAGAATACTCCGTGAAGCGACTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGTCGCTTCACGGAGTATTCTCGAGAATACTCCGTGAAGCGACTCT-3'

Other clones with same target sequence:

(none)