Construct: shRNA TRCN0000364927

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153704.5-1232s21c1
DNA Barcode:
TGACTTTCCCACTCCTATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM67 (91147)
Gene Description:
transmembrane protein 67
Transcript:
RefSeq NM_153704.5 (NON-CURRENT)
Match location:
Position 1232 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000364927)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACTTTCCCACTCCTATATT
Hairpin Sequence:
5'-CCGG-TGACTTTCCCACTCCTATATT-CTCGAG-AATATAGGAGTGGGAAAGTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACTTTCCCACTCCTATATTCTCGAGAATATAGGAGTGGGAAAGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACTTTCCCACTCCTATATTCTCGAGAATATAGGAGTGGGAAAGTCA-3'

Other clones with same target sequence:

(none)