Construct: shRNA TRCN0000365084

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182540.4-1631s21c1
DNA Barcode:
TGCCTCCATACTACCTATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
INTS6L (203522)
Gene Description:
integrator complex subunit 6 like
Transcript:
RefSeq NM_182540.4 (NON-CURRENT)
Match location:
Position 1631 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365084)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCTCCATACTACCTATTAA
Hairpin Sequence:
5'-CCGG-TGCCTCCATACTACCTATTAA-CTCGAG-TTAATAGGTAGTATGGAGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCTCCATACTACCTATTAACTCGAGTTAATAGGTAGTATGGAGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCTCCATACTACCTATTAACTCGAGTTAATAGGTAGTATGGAGGCA-3'

Other clones with same target sequence:

(none)