Construct: shRNA TRCN0000365138

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032499.4-563s21c1
DNA Barcode:
TGCTGTAGAAGGGCACATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C15orf41 (84529)
Gene Description:
chromosome 15 open reading frame 41
Transcript:
RefSeq NM_032499.4 (NON-CURRENT)
Match location:
Position 563 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365138)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGTAGAAGGGCACATAAT
Hairpin Sequence:
5'-CCGG-TGCTGTAGAAGGGCACATAAT-CTCGAG-ATTATGTGCCCTTCTACAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGTAGAAGGGCACATAATCTCGAGATTATGTGCCCTTCTACAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGTAGAAGGGCACATAATCTCGAGATTATGTGCCCTTCTACAGCA-3'

Other clones with same target sequence:

(none)