Construct: shRNA TRCN0000365163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181782.3-2830s21c1
DNA Barcode:
CAAGTTCAGTGACCACTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NCOA7 (135112)
Gene Description:
nuclear receptor coactivator 7
Transcript:
RefSeq NM_181782.3 (NON-CURRENT)
Match location:
Position 2830 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGTTCAGTGACCACTATTA
Hairpin Sequence:
5'-CCGG-CAAGTTCAGTGACCACTATTA-CTCGAG-TAATAGTGGTCACTGAACTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGTTCAGTGACCACTATTACTCGAGTAATAGTGGTCACTGAACTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGTTCAGTGACCACTATTACTCGAGTAATAGTGGTCACTGAACTTG-3'

Other clones with same target sequence:

  1. TRCN0000241622