Construct: shRNA TRCN0000365176

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015158.2-4262s21c1
DNA Barcode:
GATACTGAATGTATACGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KANK1 (23189)
Gene Description:
KN motif and ankyrin repeat domains 1
Transcript:
RefSeq NM_015158.2 (NON-CURRENT)
Match location:
Position 4262 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365176)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATACTGAATGTATACGTATT
Hairpin Sequence:
5'-CCGG-GATACTGAATGTATACGTATT-CTCGAG-AATACGTATACATTCAGTATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATACTGAATGTATACGTATTCTCGAGAATACGTATACATTCAGTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATACTGAATGTATACGTATTCTCGAGAATACGTATACATTCAGTATC-3'

Other clones with same target sequence:

(none)