Construct: shRNA TRCN0000365323

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012761.2-1084s21c1
DNA Barcode:
TGGCCACTCATAGATAATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGMB (285704)
Gene Description:
repulsive guidance molecule BMP co-receptor b
Transcript:
RefSeq NM_001012761.2 (NON-CURRENT)
Match location:
Position 1084 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365323)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCCACTCATAGATAATAAT
Hairpin Sequence:
5'-CCGG-TGGCCACTCATAGATAATAAT-CTCGAG-ATTATTATCTATGAGTGGCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCCACTCATAGATAATAATCTCGAGATTATTATCTATGAGTGGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCCACTCATAGATAATAATCTCGAGATTATTATCTATGAGTGGCCA-3'

Other clones with same target sequence:

(none)