Construct: shRNA TRCN0000365327

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018284.2-1864s21c1
DNA Barcode:
GGAACAGGCCCGAGTACTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GBP3 (2635)
Gene Description:
guanylate binding protein 3
Transcript:
RefSeq NM_018284.2 (NON-CURRENT)
Match location:
Position 1864 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365327)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAACAGGCCCGAGTACTAAA
Hairpin Sequence:
5'-CCGG-GGAACAGGCCCGAGTACTAAA-CTCGAG-TTTAGTACTCGGGCCTGTTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAACAGGCCCGAGTACTAAACTCGAGTTTAGTACTCGGGCCTGTTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAACAGGCCCGAGTACTAAACTCGAGTTTAGTACTCGGGCCTGTTCC-3'

Other clones with same target sequence:

(none)