Construct: shRNA TRCN0000365335

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022494.1-1232s21c1
DNA Barcode:
AGGAACTTTAAACAGGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZDHHC6 (64429)
Gene Description:
zinc finger DHHC-type containing 6
Transcript:
RefSeq NM_022494.1 (NON-CURRENT)
Match location:
Position 1232 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365335)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAACTTTAAACAGGTATTT
Hairpin Sequence:
5'-CCGG-AGGAACTTTAAACAGGTATTT-CTCGAG-AAATACCTGTTTAAAGTTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAACTTTAAACAGGTATTTCTCGAGAAATACCTGTTTAAAGTTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAACTTTAAACAGGTATTTCTCGAGAAATACCTGTTTAAAGTTCCT-3'

Other clones with same target sequence:

(none)