Construct: shRNA TRCN0000365349

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022826.2-2115s21c1
DNA Barcode:
AGCACACGTGTCCGATTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARCHF7 (64844)
Gene Description:
membrane associated ring-CH-type finger 7
Transcript:
RefSeq NM_022826.2 (NON-CURRENT)
Match location:
Position 2115 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365349)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCACACGTGTCCGATTTATT
Hairpin Sequence:
5'-CCGG-AGCACACGTGTCCGATTTATT-CTCGAG-AATAAATCGGACACGTGTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCACACGTGTCCGATTTATTCTCGAGAATAAATCGGACACGTGTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCACACGTGTCCGATTTATTCTCGAGAATAAATCGGACACGTGTGCT-3'

Other clones with same target sequence:

(none)