Construct: shRNA TRCN0000365360

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001114.3-3420s21c1
DNA Barcode:
GTGGCCTGATCAACGTCAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADCY7 (113)
Gene Description:
adenylate cyclase 7
Transcript:
RefSeq NM_001114.3 (NON-CURRENT)
Match location:
Position 3420 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365360)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGCCTGATCAACGTCAAAG
Hairpin Sequence:
5'-CCGG-GTGGCCTGATCAACGTCAAAG-CTCGAG-CTTTGACGTTGATCAGGCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGCCTGATCAACGTCAAAGCTCGAGCTTTGACGTTGATCAGGCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGCCTGATCAACGTCAAAGCTCGAGCTTTGACGTTGATCAGGCCAC-3'

Other clones with same target sequence:

(none)