Construct: shRNA TRCN0000365440

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173854.4-2412s21c1
DNA Barcode:
TAGCTTGGTCACTCAACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC41A1 (254428)
Gene Description:
solute carrier family 41 member 1
Transcript:
RefSeq NM_173854.4 (NON-CURRENT)
Match location:
Position 2412 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365440)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGCTTGGTCACTCAACATTT
Hairpin Sequence:
5'-CCGG-TAGCTTGGTCACTCAACATTT-CTCGAG-AAATGTTGAGTGACCAAGCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGCTTGGTCACTCAACATTTCTCGAGAAATGTTGAGTGACCAAGCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGCTTGGTCACTCAACATTTCTCGAGAAATGTTGAGTGACCAAGCTA-3'

Other clones with same target sequence:

(none)