Construct: shRNA TRCN0000365621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015262.1-378s21c1
DNA Barcode:
GTTCATAGAGAATCGTGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WASHC2C (253725)
Gene Description:
WASH complex subunit 2C
Transcript:
RefSeq NM_015262.1 (NON-CURRENT)
Match location:
Position 378 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCATAGAGAATCGTGTATA
Hairpin Sequence:
5'-CCGG-GTTCATAGAGAATCGTGTATA-CTCGAG-TATACACGATTCTCTATGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCATAGAGAATCGTGTATACTCGAGTATACACGATTCTCTATGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCATAGAGAATCGTGTATACTCGAGTATACACGATTCTCTATGAAC-3'

Other clones with same target sequence:

(none)