Construct: shRNA TRCN0000365635

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020066.4-5139s21c1
DNA Barcode:
TTTGGAGACCACGGCATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FMN2 (56776)
Gene Description:
formin 2
Transcript:
RefSeq NM_020066.4 (NON-CURRENT)
Match location:
Position 5139 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365635)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGGAGACCACGGCATATTT
Hairpin Sequence:
5'-CCGG-TTTGGAGACCACGGCATATTT-CTCGAG-AAATATGCCGTGGTCTCCAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGGAGACCACGGCATATTTCTCGAGAAATATGCCGTGGTCTCCAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGGAGACCACGGCATATTTCTCGAGAAATATGCCGTGGTCTCCAAA-3'

Other clones with same target sequence:

(none)