Construct: shRNA TRCN0000365640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019080.2-883s21c1
DNA Barcode:
TTGGCCTTTCCTTGATCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NDFIP2 (54602)
Gene Description:
Nedd4 family interacting protein 2
Transcript:
RefSeq NM_019080.2 (CURRENT)
Match location:
Position 883 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGCCTTTCCTTGATCAAAT
Hairpin Sequence:
5'-CCGG-TTGGCCTTTCCTTGATCAAAT-CTCGAG-ATTTGATCAAGGAAAGGCCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGCCTTTCCTTGATCAAATCTCGAGATTTGATCAAGGAAAGGCCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGCCTTTCCTTGATCAAATCTCGAGATTTGATCAAGGAAAGGCCAA-3'

Other clones with same target sequence:

(none)