Construct: shRNA TRCN0000365781

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207342.2-566s21c1
DNA Barcode:
TCATAATGGAACCAAGTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STEAP1B (256227)
Gene Description:
STEAP family member 1B
Transcript:
RefSeq NM_207342.2 (CURRENT)
Match location:
Position 566 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365781)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATAATGGAACCAAGTATAA
Hairpin Sequence:
5'-CCGG-TCATAATGGAACCAAGTATAA-CTCGAG-TTATACTTGGTTCCATTATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATAATGGAACCAAGTATAACTCGAGTTATACTTGGTTCCATTATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATAATGGAACCAAGTATAACTCGAGTTATACTTGGTTCCATTATGA-3'

Other clones with same target sequence:

(none)