Construct: shRNA TRCN0000365827

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080428.2-975s21c1
DNA Barcode:
CCCATGGAAGAGTGCGTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxw7 (50754)
Gene Description:
F-box and WD-40 domain protein 7
Transcript:
RefSeq NM_080428.2 (NON-CURRENT)
Match location:
Position 975 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365827)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATGGAAGAGTGCGTATAT
Hairpin Sequence:
5'-CCGG-CCCATGGAAGAGTGCGTATAT-CTCGAG-ATATACGCACTCTTCCATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATGGAAGAGTGCGTATATCTCGAGATATACGCACTCTTCCATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATGGAAGAGTGCGTATATCTCGAGATATACGCACTCTTCCATGGG-3'

Other clones with same target sequence:

(none)