Construct: shRNA TRCN0000365838

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010439.3-1184s21c1
DNA Barcode:
ATGCAGCTTATACGAAGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hmgb1 (15289)
Gene Description:
high mobility group box 1
Transcript:
RefSeq NM_010439.3 (NON-CURRENT)
Match location:
Position 1184 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000365838)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCAGCTTATACGAAGATAA
Hairpin Sequence:
5'-CCGG-ATGCAGCTTATACGAAGATAA-CTCGAG-TTATCTTCGTATAAGCTGCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCAGCTTATACGAAGATAACTCGAGTTATCTTCGTATAAGCTGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCAGCTTATACGAAGATAACTCGAGTTATCTTCGTATAAGCTGCAT-3'

Other clones with same target sequence:

(none)