Construct: shRNA TRCN0000366076

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011785.3-1330s21c1
DNA Barcode:
TGAAACAGACACCCGATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Akt3 (23797)
Gene Description:
thymoma viral proto-oncogene 3
Transcript:
RefSeq NM_011785.3 (CURRENT)
Match location:
Position 1330 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000366076)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAACAGACACCCGATATTT
Hairpin Sequence:
5'-CCGG-TGAAACAGACACCCGATATTT-CTCGAG-AAATATCGGGTGTCTGTTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAACAGACACCCGATATTTCTCGAGAAATATCGGGTGTCTGTTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAACAGACACCCGATATTTCTCGAGAAATATCGGGTGTCTGTTTCA-3'

Other clones with same target sequence:

(none)