Construct: shRNA TRCN0000366247

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027136.3-1150s21c1
DNA Barcode:
CAGACTGAAGAAGCTAGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ovca2 (246257)
Gene Description:
candidate tumor suppressor in ovarian cancer 2
Transcript:
RefSeq NM_027136.3 (CURRENT)
Match location:
Position 1150 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000366247)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGACTGAAGAAGCTAGATAT
Hairpin Sequence:
5'-CCGG-CAGACTGAAGAAGCTAGATAT-CTCGAG-ATATCTAGCTTCTTCAGTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGACTGAAGAAGCTAGATATCTCGAGATATCTAGCTTCTTCAGTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGACTGAAGAAGCTAGATATCTCGAGATATCTAGCTTCTTCAGTCTG-3'

Other clones with same target sequence:

(none)