Construct: shRNA TRCN0000366275

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177619.2-351s21c1
DNA Barcode:
CCTCGAACTCCAACCGGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kat7 (217127)
Gene Description:
K(lysine) acetyltransferase 7
Transcript:
RefSeq NM_177619.2 (NON-CURRENT)
Match location:
Position 351 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000366275)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGAACTCCAACCGGAAAT
Hairpin Sequence:
5'-CCGG-CCTCGAACTCCAACCGGAAAT-CTCGAG-ATTTCCGGTTGGAGTTCGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGAACTCCAACCGGAAATCTCGAGATTTCCGGTTGGAGTTCGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGAACTCCAACCGGAAATCTCGAGATTTCCGGTTGGAGTTCGAGG-3'

Other clones with same target sequence:

(none)