Construct: shRNA TRCN0000366468

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133215.1-2857s21c1
DNA Barcode:
TAGCAAGAGGCCGAGCAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtmr4 (170749)
Gene Description:
myotubularin related protein 4
Transcript:
RefSeq NM_133215.1 (CURRENT)
Match location:
Position 2857 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000366468)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGCAAGAGGCCGAGCAATAA
Hairpin Sequence:
5'-CCGG-TAGCAAGAGGCCGAGCAATAA-CTCGAG-TTATTGCTCGGCCTCTTGCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGCAAGAGGCCGAGCAATAACTCGAGTTATTGCTCGGCCTCTTGCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGCAAGAGGCCGAGCAATAACTCGAGTTATTGCTCGGCCTCTTGCTA-3'

Other clones with same target sequence:

(none)