Construct: shRNA TRCN0000366499

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183138.2-3075s21c1
DNA Barcode:
CGCCCACAAGGACCAACATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tet3 (194388)
Gene Description:
tet methylcytosine dioxygenase 3
Transcript:
RefSeq NM_183138.2 (CURRENT)
Match location:
Position 3075 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000366499)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCCACAAGGACCAACATAA
Hairpin Sequence:
5'-CCGG-CGCCCACAAGGACCAACATAA-CTCGAG-TTATGTTGGTCCTTGTGGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCCACAAGGACCAACATAACTCGAGTTATGTTGGTCCTTGTGGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCCACAAGGACCAACATAACTCGAGTTATGTTGGTCCTTGTGGGCG-3'

Other clones with same target sequence:

(none)